Events

12 July

Dye-loaded fluorescent polymeric nanoparticles: bright platform for biosensing and bioimaging

Thursday 12 July 2018, 04:00pm

ICN2 Seminar Hall, ICN2 Building, UAB

Andrey S. Klymchenko, ILaboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Faculté de Pharmacie, ILLKIRCH, France

Short Abstract: Fluorescent polymeric nanoparticles (NPs) appear as an attractive alternative to inorganic NPs, such as quantum dots (QDs), because of their potential biodegradability, low toxicity and high capacity to encapsulate active molecules.[1] Important current challenges with organic NPs are (i) achieving high fluorescence brightness for the smallest possible size and (ii) realizing efficient Förster resonance energy transfer (FRET) from NPs, which are normally larger than the Förster radius. We showed that the small size of NPs can be achieved by using nanoprecipitation of polymers bearing 1-2 charged groups per chain.[2,3] To achieve high NPs brightness, we designed cationic dyes (rhodamines and cyanines) containing large fluorinated counterions that prevent aggregation caused self-quenching of dyes (Figure 1).[2,4] As a result, we obtained PLGA and PMMA NPs of 15-60 nm encapsulating 100-10000 of dyes, which are many-fold brighter than QDs. Moreover, inside NPs, dyes showed remarkable collective behavior, observed as particle on/off switching, due to ultrafast exciton migration.[2,5] This collective behavior of dyes was successfully applied to obtain giant light-harvesting nanoantenna that amplifies ~1000-fold emission of single FRET acceptor dyes and enables first single-molecule detection in ambient sunlight conditions.[5] This organic nanoantenna was successfully applied for amplified fluorescence detection of biomolecules. Moreover, using NPs encapsulating cyanine dyes, we developed new methodology for long-term color coding of living cells in vitro and in vivo.[6]

References

  1. Reisch, A.; Klymchenko, A.S. Small, 2016, 12 , 1968.
  2. Reisch, A.; Didier, P.; Richert, L.; Oncul, S.; Arntz, Y.; Mely, Y.; Klymchenko, A. S. Nat. Commun. 2014, 5, 4089.
  3. Reisch, A.; Runser, A.; Arntz, Y.; Mély, Y.; Klymchenko, A.S. ACS Nano, 2015, 9, 5104.
  4. Shulov, I.; Oncul, S.; Reisch, A.; Arntz, Y.; Collot, M.; Mely, Y.; Klymchenko, A. S. Nanoscale, 2015, 7, 18198.
  5. Trofymchuk, K., Reisch, A., Didier, P. Fras, F., Gilliot, P. Mely, Y. Klymchenko, AS, Nature Photonics, 2017, 11, 657.
  6. Andreiuk B, Reisch A, Lindecker M, Follain G, Peyriéras N, Goetz JG, Klymchenko AS, Small, 2017, 13, 1701582.

 

Hosted by: CSIC Tenured Scientist Daniel Ruiz-Molina - Nanostructured Functional Materials Group Leader